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ScienCell
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BioWhittaker Molecular Applications
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HiMedia Laboratories
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STEMCELL Technologies Inc
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BioWhittaker Molecular Applications
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Poietics Inc
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Image Search Results
Journal: Frontiers in Endocrinology
Article Title: Extracellular Vesicles Inhibit Proliferation and Invasion of Ovarian Endometrial Stromal Cells and Their Expression of SF-1, ERβ, and Aromatase
doi: 10.3389/fendo.2021.666195
Figure Lengend Snippet: Identification of UC-MSCs. (A) UC-MSCs were exposed to adipogenic differentiation medium and stained by Oil Red O. Compared to cells in the control group, the red, round lipid droplets observed in UC-MSCs treated with adipogenic differentiation medium confirms their differentiation into adipocytes. (B) UC-MSCs were cultured with osteoblast differentiation medium and stained by Alizarin Red S. As evidenced by the red round droplet in the righthand image, UC-MSCs could differentiate into osteocytes. (C) Stem cell phenotypes were identified by flow cytometry. Positive indicators (CD90, CD105, CD44, and CD29) showed that the cells to be tested were stem cells rather than ordinary cells. In addition, negative markers (CD19 and HLA-DR) showed that the cells to be tested were mesenchymal stem cells rather than hematopoietic stem cells. All experiments were repeated three times.
Article Snippet: MSCs were seeded in 6-well plates at 10 5 cells/well, and then cultured in
Techniques: Staining, Control, Cell Culture, Flow Cytometry